Atelier de production d’embryons de bovins

L’atelier produit des embryons in vitro, après maturation in vitro des ovocytes, fécondation in vitro ou transfert nucléaire (Clonage). Les embryons sont cultivés in vitro jusqu'au stade requis pour les analyses ultérieures.

Techniques mises en œuvre

Maturation d’ovocytes in vitro, fécondation in vitro, culture d’embryons, clonage par transfert de noyau, suivi morphocinétique des embryons, biopsies, congélation.

Quelques chiffres /année

• 13000 ovocytes collectés
• 2100 blastocystes obtenus
• 230 blastocystes transférés

Bovins clonés obtenus après transferts d'embryons clonés au sein de l'atelier "Production d'embryons bovins" de l'unité BDR.

Publications

2017

Lelievre, J-M., Peynot, N., Ruffini, S., Laffont, L., Le Bourhis, D., Girard, P-M., Duranthon, V., 2017. Regulation of heat-inductible HSPA1A gene expression during maternal-to embryo transition and in response to heat in in vitro-produced bovine embryos. Reprod Fertil Dev, 29 (9): 1868-1881 https://www.ncbi.nlm.nih.gov/pubmed/27851888

Nuttinck, F., Jouneau, A., Charpigny, G., Hue, I., Richard, C., Adenot, P., Ruffini, S., Laffont, L., Chebrout, M., Duranthon, V., Marquant-Le Guienne, B., 2017. Prosurvival effect of cumulus prostaglandin G/H synthase 2/prostaglandin 2 signaling on biovine blastocyst: impact on in vivo posthatching development. Biol Reprod, 96 (3): 531-541 https://www.ncbi.nlm.nih.gov/pubmed/28339853

Vitorino Carvalho, A., Canon, E., Jouneau, L., Archilla, C., Laffont, L., Moroldo, M., Ruffini, S., Corbin, E., Mermillod, P., Duranthon, V., 2017. Different co-culture systems have the same impact on bovine embryo transcriptome. Reproduction,154(5):695-710 https://www.ncbi.nlm.nih.gov/pubmed/28982934

2016

Biase FH., Rabel, C., Guillomot, M., Hue, I., Andropolis, K., Olmstead, CA., Oliveira, R.,  Wallace, R., Le Bourhis, D., Richard, C., Campion, E., Chaulot-Talmon, A., Giraud-Delville, C., Taghouti, G., Jammes, H., Renard, JP, Sandra, O., Lewin, HA., 2016. Massive dysregulation of genes involved in cell signalign and placental development in cloned cattle conceptus and maternal endometrium. PNAS, 113 (51): 14492-14501 https://www.ncbi.nlm.nih.gov/pubmed/27940919

Kiefer, H., Jouneau, L., Campion, E., Rousseau-Ralliard, D., Larcher, T., Martin-Magniette, M-L, Balzergue, S., Ledevin, M., Prézelin, A., Chavatte-Palmer, P., Heyman, Y., Richard, C., Le Bourhis, D., Renard, J-P., Jammes, H., 2016.  Altered DNA methylation associated with an abnormal liver phenotype in a cattle model with a high incidence of perinatal pathologies. Sci Rep- UK, 6: e38869 https:///www.ncbi.nlm.nih.gov/pubmed/27958319

Sepulveda-Rincon, LP., Dube, D., Adenot, P., Laffont, L., Ruffini, S., Gall , L., Campbell, BK., Duranthon, V., Beaujean, N., Maalouf, WE., 2016. Random allocation of blastomere descendants to the trophectoderm and ICM of the bovine blastocyst. Biol Reprod, 95 (6): 123, 1-10 https:///www.ncbi.nlm.nih.gov/pubmed/27760750

2014

Boulanger, L., Pannetier, M., Gall, L., Allais-Bonnet, A., Elzaiat, M., Le Bourhis, D., Daniel, N., Richard, C., Cotinot, C., Ghyselinck, N.B., Pailhoux, E., 2014. FOXL2 is a female sex–determining gene in the goat. Curr Biol, 24 (4): 404-408 https://www.ncbi.nlm.nih.gov/pubmed24485832

Guillomot, M., Campion, E., Prézelin, A., Sandra, O., Hue, I., Le Bourhis, D., Richard, C., Biase, F.H., Rabel, C., Wallace, R., Lewin, H., Renard, J-P., Jammes, H., 2014. Spatial and temporal changes of Decorin, Type I Collagen and Fibronectin expression in normal and clone bovine placenta. Placenta, 35 (9): 737-747 https://www.ncbi.nlm.nci.gov/pubmed/25012297

Vittorino Carvalho, A., Reinaud, P., Forde, N., Healey, G.D.,  Eozenou, C., Giraud-Delville, C., Mansouri-Attia, N., Gall, L., Richard, C., Lonergan, P., Sheldon, IM., Lea, RG., Sandra, O., 2014. SOCS genes expression during physiological and perturbed implantation in bovine endometrium. Reproduction, 148: (6): 545-557 https://www.ncbi.nlm.nih.gov/pubmed/24813750

2013

Biase, F. H., Rabel, C., Guillomot, M., Sandra, O., Andropolis, K., Olmstead, C., Oliveira, R., Wallace, R., Le Bourhis, D., Richard, C., Campion, E., Chaulot-Talmon, A., Giraud-Delville, C., Taghouti, G., Jammes, H., Hue, I., Renard, J-P., Lewin, H. A., 2013. Changes in WNT signaling-related gene expression associated with development and cloning in bovine extra-embryonic and endometrial tissues during the peri-implantation period. Mol Reprod Dev, 80 (12): 977-987 https://www.ncbi.nlm.nih.gov/pubmed/24038527